Emily Mihalko, PhD

Education & Training

• Ph.D.: North Carolina State University & University of North Carolina (Raleigh, NC)

Publications:

1. Mihalko EP, Kar R, Holthaus B, Rassam R, Sharma R, Wheeler D, Nimjee SM, Shea SM. Von Willebrand Factor A1 Blockade Prevents Platelet-Mediated Sustained Occlusion for the Treatment of Arterial Thrombosis. Commun Biol. 2026 Jul 11;9(1):1024. PMID: 42436238.
2. Killinger J, Alsaadi N, Younes R, Hoteit L, Faine B, Mohr N, Puccio AM, McIntyre P, Raymond O, Filicky A, Hahner T, Agnone AG, Kaplan A, Vincent LE, Srinivasan A, Dishong DM, Abdullah A, Arivudainambi A, Kar R, Mihalko EP, Loughran P, Wisniewski S, Guyette F, Luther JF, Spinella PC, Okonkwo D, Sperry J, Shea S, Neal MD. Platelet function assays fail to detect differences between transfusion of cold or room temperature platelets in traumatic brain injury patients. J Trauma Acute Care Surg. 2026 Apr 1;100(4):555-561. PMID: 41575197.
3. Mihalko EP, Munitz R, Dishong DM, Clayton S, Shea SM. Microfluidic transection injury and high shear thrombus formation demonstrate increased hemostatic efficacy of cold-stored platelets and in vitro resuscitation in induced coagulopathy models. Frontiers in Bioengineering and Biotechnology. Front Bioeng Biotechnol. 2025 May 12:13:1568113. PMID: 40421116.
4. Alsaadi N, Younes R, Killinger J, Hoteit L, Puccio AM, McIntyre P, Raymond O, Filicky A, Hahner T, Agnone AG, Vincent LE, Srinivasan A, Zarisfi M, Dishong D, Abdullah A, Arivudainambi A, Kar R, Mihalko EP, Loughran P, Wisniewski S, Luther JF, Okonkwo D, Sperry J, Shea S, Neal MD. Predictive value of platelet function assays in traumatic brain injury patients on antiplatelet therapy. J Trauma Acute Care Surg. 2025 Apr 1;98(4):550-556. PMID: 39956975.
5.  Andraska EA, Denorme F, Kaltenmeier C, Arivudainabi A, Mihalko EP, Dyer M, Annarapu G, Zarisfi M, Loughran P, Ozel M, Williamson K, Mota Alvidrez RI, Thomas K, Shiva S, Shea SM, Steinman RA, Campbell RA, Rosengart MR, Neal MD. Alterations in visible light exposure modulate platelet function and regulate thrombus formation. J Thromb Haemost. 2025 Jan; 23(1):123-138. PMID: 39299611.
6. Mihalko EP, Abdullah A, Hoteit L, Akinbode D, Shea S, Neal MD. Microfluidics in Assessing Platelet Function. J Vis Exp. 2024 Nov 8:(213). PMID: 39584679.
7. Sheridan A, Nellenbach K, Pandit S, Byrnes E, Hardy G, Moiseiwitsch N, Scull G, Mihalko EP, Levy JH, Brown AC. Clot-targeted nanogels for dual-delivery of antithrombinIII and tissue plasminogen activator to mitigate disseminated intravascular coagulation complications. ACS Nano. 2024 Jun;18(24):15517-15528. PMID: 38836363.
8. Simpson A*, Mihalko EP*, Fox C, Sridharan S, Krishnakumar M, Brown AC. Biomaterial systems for evaluating the influence of ECM mechanics on anti-fibrotic therapeutic efficacy. Matrix Biology Plus. 2024 May 25:23:100150. PMID: 38882395
9. Krissanaprasit A, Mihalko EP, Meinhold K, Simpson A, Dupont DM, Kjems J, Brown AC, LaBean TH. A Functional RNA-Origami as Direct Thrombin Inhibitor with Fast-acting and Specific Single-Molecule Reversal Agents in vivo model. Molecular Therapy. 2024 May 7:S1525-0016(24)00302-2. PMID: 38720458.
10. Nellenbach K*, Mihalko EP*, Nandi S*, Koch D, Shetty J, Sollinger J, Moiseiwitsch N, Sheridan A, Schnabel LV, Lyon A, Barker TH, Brown AC. Ultrasoft platelet-like particles stop bleeding in rodent and porcine models of trauma. Science Translational Medicine. 2024 Apr 10; 16(742):eadi4490. PMID: 38598613.
11. Shea SM, Reisz JA, Mihalko EP, Rahn KC, Rassam RMG, Chitrakar A, Gamboni F, D’Alessandro A, Spinella PC, Thomas KA. Cold-stored platelet hemostatic capacity is maintained for three weeks of storage and associated with taurine metabolism. J Thromb Haemost. 2024 Apr; 22(4):1154-1166. PMID: 38072374.
12. Shea SM, Mihalko EP, Lu L, Thomas KA, Schuerer D, Brown JB, Bochicchio GV, Spinella PC. Doing more with less: low-titer group O whole blood resulted in less total transfusions and an independent association with survival in adults with severe traumatic hemorrhage. J Thromb Haemost. 2024 Jan; 22(1):140-151. PMID: 37797692.
13. Chee E, Mihalko EP, Nellenbach K, Sollinger J, Huang K, Hon M, Cheng K, Brown AC. Wound-triggered Shape Change Microgels for the Development of Enhanced Biomimetic Function Platelet-like Particles. J Biomed Mater Res A. 2024 Apr; 112(4):613-624. PMID: 37846887.
14. Mihalko EP*, Srinivasan AJ*, Rahn KC, Seheult JN, Spinella PC, Cap AP, Triulzi DJ, Yazer MH, Neal MD, Shea SM. Hemostatic In Vitro Properties of Novel Plasma Supernatants Produced from Late-Storage Low-Titer Type O Whole Blood. Anesthesiology. 2023 Jul 1; 139(1):77-90. PMID: 37027803.
15. Shea SM, Thomas KA, Rassam R, Mihalko EP, Daniel C, Sullenger BA, Spinella PC, Nimjee SM. Dose-Dependent Von Willebrand Factor inhibition by Aptamer BB-031 Correlates with Thrombolysis in a Microfluidic Model of Arterial Occlusion. Pharmaceuticals. 2022 Nov 22; 15(12):1450. PMID: 36558901.
16. Mihalko EP, Nellenbach K, Krishnakumar M, Moiseiwitsch N, Sollinger J, Cooley BC, Brown AC. Fibrin-specific poly(N-isopropylacrylamide) nanogels for targeted delivery of tissue-type plasminogen activator to treat thrombotic complications are well tolerated in vivo. Bioeng Transl Med. 2021 Dec 11; 7(2):e10277. PMID: 35600656.
17. Nandi, S., Mihalko, E., Nellenbach, K., Castaneda, M., Schneible, J., Menegatti, S., Barker, T., Brown, A. Synthetic platelet microgels containing fibrin B knob mimetic motifs enhance clotting responses. Advanced Therapeutics. (2021). doi.org/10.1002/adtp.202100010.
18. Todd, Jordan; Bharadwaj, Vimala; Nellenbach, Kimberly; Nandi, Seema; Copeland, Connor; Mihalko, Emily; Brown, Ashley; Stabenfeldt, Sarah. Platelet-Like Particles Reduce Coagulopathy-Related and Neuroinflammatory Pathologies Post-Experimental Traumatic Brain Injury. Journal of Biomedical
19. Mihalko, E.P., Sandry, M., Mininni, N., Nellenbach, K., Deal, H., Daniele, M., Ghadimi, K., Levy, J.H., Brown, A.C. Fibrin-modulating nanogels for treatment of disseminated intravascular coagulation. Blood Advances. (2021). doi.org/10.1182/bloodadvances.2020003046.
20. Abhichart Krissanaprasit, Carson Key, Kristen Froehlich, Sahil Pontula, Emily Mihalko, Daniel Miotto Dupont, Ebbe Sloth Andersen, Jørgen Kjems, Ashley C. Brown, and Thomas H. LaBean. Multivalent Aptamer-Functionalized Single-Strand RNA Origami as Effective, Target-Specific Anticoagulants with Corresponding Reversal Agent. Advanced Healthcare Materials. (2020). doi:10.1002/adhm.202001826
21. Nandi, S., Sommerville, L., Nellenbach, K., Mihalko, E., Erb, M., Freytes, D.O., Hoffman, M., Monroe, D., Brown, A.C. Platelet-like particles improve fibrin network properties in a hemophilic model of provisional matrix structural defects. Journal of Colloid and Interface Science. (2020).
    doi.org/10.1016/j.jcis.2020.05.088.